Warm greeting to all of you!
Its my pleasure to introduce myself. My name is Sandeep Dhiman a professional microbiologist and consultant for microbiology. I am post graduate and love to share knowledge with people. I have hands on experience in microbiological concepts, testing procedures and validations etc. I am very happy to share my knowledge through this blog which I created for pharmaceutical professionals.
Its my pleasure to introduce myself. My name is Sandeep Dhiman a professional microbiologist and consultant for microbiology. I am post graduate and love to share knowledge with people. I have hands on experience in microbiological concepts, testing procedures and validations etc. I am very happy to share my knowledge through this blog which I created for pharmaceutical professionals.
sir pls provide me complete information about water purification system step by step from source water to wfi.
ReplyDeleteHello Sandeep, thanks for your articel about 0.45 micron and 0.22 filtration. I am confused about the difference between 0.22 and 0.2 filters. Please, can you tell me where can I look for it?
ReplyDeleteThanks
Chris
Hello Sandeep, pls i need to develop competency protocol and also write an SOP for microbiology competency, how do i go about it.
ReplyDeleteThanks
Why pseudomonas is used for microbial challenged test
ReplyDeleteWhy pseudomonas is used for microbial challenged test
ReplyDeleteDear Rupesh,
ReplyDeletePlease look into USP chapters for any method Suitability test for MLT, GPT and sterility. It's not only Pseudomonas aeruginosa , but a list of other organisms as well which is representative of gram positive and negative, aerobic and anaerobic, yeasts and mould.
Another reason is that these are the list or representative of Specified organisms which should not be present in an pharmaceutical substance or product.
S.Ravi.
Chennai.
In Microbiology, container closure integrity test is performed with final inoculum concentration of B.diminuta must be 10 6.Why is in 10 6 only y cant we use 10 7 or 10 5 culture.
ReplyDeleteRaguvarman Mahadevan
In Microbiology, container closure integrity test is performed with final inoculum concentration of B.diminuta must be 10 6.Why is in 10 6 only y cant we use 10 7 or 10 5 culture.
ReplyDeleteRaguvarman Mahadevan
Sir.how much concentration of neutraling agent used for sample.
ReplyDeleteIt is only depend upon your validation study. You need to perform validation to calculate the required concentration or volume of neutralizing agent.
DeleteDear sir ,how can perform environment Monitoring program in dynamic pass box as u.v light is continuously on in the pass box
ReplyDeleteDear Friend, exposing plates in dynamic pass box under UV light is not a good idea because you'll not get actual results as UV light is bactericidal.So, either you do it in UV off condition or just perform volumetric air sampling and surface monitoring in dynamic pass box.
DeleteKeep it bro. You are doing great work.
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tank u
ReplyDeleteHi Sandip, I have a question about environmental monitoring by single media (SCDA) and dual temperature incubation. How authentic taking fungal count in this case separately, when the media is not specific to fungi? Because in this case we are identifying the fungal count only morphologically? Taking Total count is understandable, but how do we justify reporting fungal count as well?
ReplyDeleteSCDA media is considered as general purpose media which is used for the growth of large number of bacteria and fungus. In environment monitoring basically SCDA media is used but if you want to do the monitoring to identify fungus only then you can use SDA media at a defined frequency to isolate fungus. You can defined the frequency of monthly or quarterly as per your risk assessment.
DeleteThanks