Differences between endotoxin and exotoxin

There are so many differences between endotoxin and exotoxin. Endotoxin are released by gram negative bacterial cell from their cell wall and released only after the cell lysis. Living gram negative bacteria can't release endotoxin. That's why they are called endotoxin.

Exotoxin are mainly produced by the gram positive bacteria and released outside the cell by living bacterial cell. That's why they are called exotoxin. Differences between both are mentioned below.

Properties	Endotoxin	Exotoxin
Chemical Nature	Lipopolysaccharides	Proteins
Molecular weight	10 k Da	50-1000 k Da
Effect of heat	Heat stable not denatured	Denatured by heat
Relationship to cell	Part of outer membrane	Extracellular
Specificity	Low	High
Pyrogenicity	Yes	Occasionally
Source	Gram negative bacteria like E.coli, P.aeruginosa, Salmonella	Commonly gram positive bacteria like S.aures, B.subtilis
Can be toxoid	No	Yes
Antigenicity	Poor	Very good
Potency	Low	High
Mode of detection	Limulus amebocyte lysate	Neutralization, Precipitation etc.

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