Subculturing is one of the method to transfer and preserve the microbial culture for long time. But how many transfers are acceptable, this is a question of importance and what happens if we exceed the recommended number of transfers? First we have to understand the meaning of passage. Passage is define as the transfer of microorganisms from viable culture to fresh medium. Every transfer is called passage whether we transfer the culture from solid media to solid media or solid media to liquid media. As per pharmacopoeia, the microorganisms involved in the test should not be five passage removed from the orignal culture which means we can only transfer the culture up to five times into fresh medium from the orignal culture. Next question comes why not more than five passages? If we go for more than five passages, there might be chances of phenotypic changes or mutation in the culture. To prevent such type mutation not more than five passages are recommended.
Thanks and Have a great day!
Thanks and Have a great day!
Tnx a lot
ReplyDeletethank u sir one dought is there one lyophilized passage no2 culture transfer in to the liquid media what is that passage ?
ReplyDeleteEvery transfer is counted as passage whether you transfer in liquid medium or solid medium. So, if you have lyophilized culture of 2nd passage and after transferring in liquid medium this can be considered as 3rd passage.
DeleteThanks
Dear Chandra Mohan, every transfer into fresh medium (liquid or solid) shall be counted as passage.
ReplyDeleteSir which will be our first passage from cultiloop to fresh medium (mother culture) or from mother culture to fresh medium?
ReplyDeleteWhat will be the time span between two passage?
What bet test
ReplyDelete