Saline solution is used for the preparation of culture suspension or serial dilution in microbiology. Saline is 0.9 % sodium chloride solution. Culture suspensions are prepared in saline solution because 0.9 % sodium chloride solution is isotonic in nature. In isotonic solution the concentration of solutes remains the same both inside and outside of the microbial cell and cells remains at their usual osmotic pressure. But water is hypotonic in nature in which the concentration of solute is greater inside the cell than outside and due to this water try to enter inside the bacterial cell. The osmotic pressure develop on the bacterial cell which can damage the bacterial cells during serial dilution. That's why serial dilution is prepared in 0.9% saline solution.
Thanks and have a nice day!
If negative control fails what to do? and how investigation to be done sir? please explain
ReplyDeleteIf negative control fails, it means there might be problem with the testing environment, handling problem or problem in the accessories used for the analysis. In that case investigation shall be done why negative control failed? You can investigate with check list or using different tools like why why analysis or fish bone diagram.
DeleteIn which condition hypertonic saline is used for dilution in place of normal saline? And why?
DeleteWhy most of the bacterial colony on the agar surface is circular????
ReplyDeleteWhy most of the bacterial colony on the agar surface is circular????
ReplyDeleteMay I know which solution can be used as buffer or maintaining osmotic pressure for serial dilution based isolation of fungi or specifically fungal endophytes from plant tissues?
ReplyDeletecould you explian why we are keeping microbial plates in inverted position?
ReplyDeleteTo prevent contamination
Deletecould you explian why we are keeping microbial plates in inverted position?
ReplyDeleteIf v keep the plates in upright position in incubator then water content from agar get evaporated and get accumulated on the lid of the plate in form of vapours. These vapours get condensed and again fall down on agar plate.. this may cause contamination and if there are some Microbial colonies on the plate then they got spreaded due to condensed water droplet fallen down on colony surface. Datz why v r incubated the media plates in inverted position.
Deletewe are using scdm for the serial dilution is it OK.
ReplyDeleteHow do you check the pathogenicity of bacteria?
ReplyDeleteIts v simple way to test, reinoculate the isolated bacterial strain on its host, second way to extract the genomic DNA of bacterial strain and amplify the pathogenic genes which are responsible for production of pathogenic compounds/symptoms for cause the disease
DeleteUseful information.
ReplyDelete